Addressing the ever-growing applications of flow cytometry in such areas as oncology, pathology, biotech, pharmaceuticals, and oceanography, the third edition of this well-regarded, highly readable book introduces a new generation of scientists, technicians, and physicians to the state of the art in flow cytometry. Covering both principles and practice from the ground up, the new edition brings all topics up to date, examining the latest advances in flow cytometer equipment, as well as new methods for storing flow data, newly available fluorochromes, sorting of cells, clinical flow cytometry, and much more.

Table of Contents

Preface.

Acknowledgments (First Edition).

Acknowledgments (Second Edition).

The Past as Prologue.

Setting the Scene.

Instrumentation: Into the Black Box.

Information: Harnessing the Data.

Seeing the Light: Lasers, Fluorochromes, and Filters.

Cells from Without: Leukocytes, Surface Proteins, and the Strategy of Gating.

Cells from Within: Intracellular Proteins.

Cells from Within: DNA in Life and Death.

The Sorting of Cells.

Disease and Diagnosis: The Clinical Laboratory.

Out of the Mainstream: Research Frontiers.

Flowing On: The Future.

General References.

Glossary.

Figure Credits.

Index.