Reseña o resumen
DNA sequencing techniques have evolved rapidly in recent years largely resulting from the Human Genome Project but scientists still encounter challenges in sequencing genomes. DNA Sequencing: Optimizing the Process and Analysis was written to help scientists sequence difficult templates. It is also a practical guide to faster and more efficient routine DNA sequencing. This in-depth volume covers important topics in the field, including biochemical and technological advances stemming from the Human Genome Project, proven and newly emerging methods of preparing DNA templates, the effects of widely used laboratory reagents on DNA sequencing, Laboratory Information Management Systems (LIMS), setting up of a GLP/GMP DNA sequencing lab, and the future of DNA sequencing.
Brief Table of Contents
Foreword by Elaine Mardis
Chapter 1: Controlled Heat-Denaturation of DNA Plasmids
Jan Kieleczawa, Wyeth Research
Chapter 2: Effect of Various Reagents on DNA Sequencing
Jan Kieleczawa, Wyeth Research
Chapter 3: Sequencing of Difficult DNA Templates
Jan Kieleczawa, Wyeth Research
Chapter 4: New DNA Sequencing Enzymes
Sandra L. Spurgeon and John W. Brandis, Applied Biosystems
Chapter 5: Beyond pUC: Vectors for Cloning Unstable DNA
Ronald Godiska, Melodee Patterson, Tom Schoenfeld, and David A. Mead, Lucigen Corporation, Middleton, WI
Chapter 6: Recombination-Based Cloning
Vincent Ling, Wyeth Research
Chapter 7: Plasmid Preparation Methods for DNA Sequencing
Parke K. Flick, Amersham Biosciences
Chapter 8: Optimization of Culture Growth in 96-Deep-Well Plates
Jan Kieleczawa, Wyeth Research
Chapter 9: Automated DNA Scanners Used in Sequencing Laboratories
Jan Kieleczawa, Wyeth Research
Chapter 10: Geospiza's Finch-Server: A Complete Data Management System for DNA Sequencing
Sandra Porter, Joe Slagel, and Todd Smith, Geospiza, Inc.
Chapter 11: DNA Sequencing Database: A Flexible LIMS for DNA Sequencing Analysis
Donald M. Koffman, DMK Concepts Inc. and Hemchand Sookdeo, Wyeth Research
Chapter 12: Good Laboratory Practices, Good Manufacturing Procedures, and Quality Assurance in the DNA Sequencing Laboratory
Michele Godlevski and Thalia Taylor, GlaxoSmithKline
Chapter 13: The Future of DNA Sequencing: Towards an Affordable Genome
Kevin McKernan, Agencourt Biosciences