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Morphology Methods "Cell and Molecular Biology Techniques"

193.5
183.83
Reviews: The book nicely covers the hot topics in this area -- laser capture microdissection, in situ hybridization, FISH, tyramide amplification methods, etc. An attractive feature of this book is that the last 10 chapters (of the 20) contain actual "cookbook" protocols (e.g., PCR, confocal laser scanning microscopy, immunohistochemistry, antigen retrieval, etc.) that can be adapted immediately to any laboratory. . .This book would be useful for those needing a concise reference for how to perform modern molecular tissue-based technology. -Doody's Health Science Book Review Journal

Table of Contents

Introduction to Molecular Methods, Ricardo V. Lloyd. Laser Capture Microdissection: Principles and Applications, Ricardo V. Lloyd (Protocols: H&E Staining, Operating the PIX Cell System; DNA Extraction from Collected Cells; RNA Extraction from Collected Cells). In Situ Hybridization: Detection of DNA and RNA, Long Jin, Xiang Qian, and Ricardo V. Lloyd (Protocol: In Situ Hybridization). In Situ Detection of Infectious Agents, Gary W. Procop and Randall Hayden (Protocols: Detecting Adenovirus, Epstein-Barr Virus). Fluorescent In Situ Hybridization (FISH), Susan Sheldon (Protocol: FISH Materials). Practical Applications of the FISH Technique, George Kontogeorgos, Nikiforos Kapranos, and Eleni Thodou (Protocols: Metaphase Analysis from Whole Blood Culture; Metaphase Analysis from Solid Tumors; Interphase Analysis on Cell Imprints-Cytology Specimens; Interphase Analysis of Formalin-Fixed Paraffin-Embedded Tissues). Tyramide Amplification Methods for In Situ Hybridization, John L. Frater and Raymond R. Tubbs (Protocol: TSA, 1995; CARD/TSA with Nanogold; Tyramide-enhanced FISH with Peroxidase-labeled Oligonucleotides). Protocols: Ultrastructural In Situ Hybridization, Akira Matsuno, Tadashi Nagashima, R. Yoshiyuki Osamura, and Keiichi Watanabe. Quantitation of In Situ Hybridization Analysis, Lars-Inge Larsson. Protocols: Confocal Laser Scanning Microscopy, Akira Matsuno, Johbu Itoh, Tadashi Nagashima, R. Yoshiyuki Osamura, and Keiichi Watanabe. Protocols: Polymerase Chain Reaction, Yuri E. Nikiforov and Philip N. Howles. Protocols: Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase Chain Reaction, Omar Bagasra, Lisa E. Bobroski, and Muhammad Amjad. Immunohistochemistry: Theory and Practice, Patrick C. Roche and Eric D. Hsi. Antigen Retrieval Technique for Immunohistochemistry: Principles, Protocols, and Further Development, Clive R. Taylor and Shan-Rong Shi (Protocol: Antigen Retrieval (AR) Technique). Tyramide Amplification in Immunohistochemistry, Naoko Sanno, Akira Teramoto, and R. Yoshiyuki Osamura (Protocol: CARD Immunohistochemistry). Application of Immunohistochemistry in the Diagnosis of Lymphoid Lesions, Paul J. Kurtin. Applications of Immunohistochemistry in the Diagnosis of Undifferentiated Tumors, Mark R. Wick and Lisa A. Cerilli. Applications of Immunohistochemistry in the Diagnosis of Endocrine Lesions, Ricardo V. Lloyd. Ultrastructural Immunohistochemistry, Sergio Vidal, Eva Horvath, and Kalman Kovacs (Protocol: Ultrastructural Immunohistochemistry). Protocols: Clonality Analysis, Aurel Perren and Paul Komminoth. Index.

Autores
ISBN
978-0-89603-955-1
EAN
9780896039551
Editor
Humana Press
Stock
NO
Idioma
Inglés
Nivel
Profesional
Formato
Encuadernado
Tapa Dura
Páginas
438
Largo
-
Ancho
-
Peso
-
Edición
Fecha de edición
01-01-2001
Año de edición
2001
Nº de ediciones
1
Colección
-
Nº de colección
-